Glow Chemiluminescence Microplate Reader HM--96C

Product Overview

The HM--96C Glow Chemiluminescence Microplate Reader is dedicated to demanding quantitative bioassays where ultra-low optical background and high signal-to-noise ratio are paramount. In glow-type chemiluminescence assays, target biomolecules bind specifically to enzyme-labeled antibodies or probes, catalyzing a sustained chemical redox reaction that produces stable photon output over an extended duration. Because no excitation lamp or laser is involved, light scattering and optical autofluorescence are effectively eliminated.

Designed to accelerate bioassay workflows across biochemistry, pharmacology, and agricultural inspection, the HM--96C pairs high-throughput microplate transport with advanced detection optics. Operators can run both flash-free end-point and kinetic top-reading protocols on standard 96-well plates (50 to 200 μL/well) and high-density 384-well plates (20 to 50 μL/well). The combination of low optical crosstalk (≤0.01%), high dynamic range (7 logs), and integrated software workflow customization ensures rigorous data repeatability for complex biological matrices.

Key Features

• Glow Chemiluminescence Technology: Measures sustained photon release from enzyme-catalyzed reactions without excitation light, completely avoiding autofluorescence and stray background light.
• Imported Photon-Counting PMT Detector: High-sensitivity photomultiplier tube directly captures discrete photon events to achieve a low detection limit of 50 pg/mL and a 7-log dynamic range.
• Wide Spectral Range with User-Changeable Filters: Broad optical coverage from 230 to 700 nm with standard 470 nm and 560 nm filter modules, supporting wavelength customization for diverse luminescent substrates.
• Ultra-Low Inter-Well Crosstalk: Precision optical isolation design limits signal crosstalk between adjacent wells to ≤0.01% for 96-well plates and ≤0.05% for 384-well plates, preventing false-positive bleed-through.
• Dual Microplate Compatibility: Seamlessly accommodates both 96-well plates (50–200 μL/well) and 384-well high-density microplates (20–50 μL/well) for versatile throughput requirements.
• Flexible Reading Modes: Offers top-reading end-point measurement and time-resolved kinetic curve tracking to evaluate reaction rates and binding dynamics.
• Built-in Incubation and Linear Shaking: Integrated thermal regulation from ambient to 45°C ensures temperature stability for enzyme kinetics, combined with linear orbital shaking for thorough reagent mixing.
• Intelligent PC Software and Workflow Builder: Comprehensive Windows 10/11 analytical software provides customizable workflow protocols, multi-model curve fitting, and automated tabular data export.

Applications

• Biological Research: Quantitative total protein determination, specific enzyme activity kinetics, gene expression reporter assays (e.g., luciferase assays), and cell viability testing.
• Pharmaceutical Development: High-throughput drug candidate screening, enzyme inhibition assays, pharmacokinetic profiling, and pharmaceutical purity evaluation.
• Clinical and Diagnostic Testing: Immunoassays for tumor markers, infectious disease antigens and antibodies, metabolic hormone profiling, and early pathology biomarker screening.
• Agricultural and Veterinary Medicine: Rapid screening of animal infectious diseases, veterinary drug residue monitoring, and crop pathogen diagnostic assays.
• Food Safety Inspection: High-sensitivity detection of pathogenic microorganisms, food allergens, illegal chemical additives, and mycotoxins in food products and raw ingredients.
• Environmental Monitoring: Trace quantification of organic pollutants, toxic residues, and environmental endocrine-disrupting chemicals in water bodies and soil extracts.

Technical Specifications

Product Enquiries

Contact our sales representative for product enquiries, configuration information and coordination of service requests.

Sales Contact: Grace
Email: graceliu@hmdzkj.cn
Tel / WhatsApp / WeChat: +86 17853698681