Avian Disease Detector HM-P08

The avian disease detector is a device used to detect potential pathogens such as Infectious Bursal Disease Virus (IBDV), Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), avian leukosis, Avian Influenza Virus (AIV), Duck Plague Virus (DPV), and Gosling Plague in poultry (chickens, ducks, geese, etc.). These detection instruments are primarily used to monitor the health status of poultry and to detect and control the spread of poultry diseases early.

Real-time PCR detection combines PCR and fluorescence detection technologies. Fluorescence PCR detectors can monitor the progress of the amplification reaction in real-time during the PCR process, providing more rapid and accurate detection results. It is widely used in the poultry farming industry and veterinary health fields.

Basic Principles 

Principle of real-time quantitative PCR instrument: Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA amplification outside the organism. It consists of several steps including high-temperature denaturation, low-temperature annealing, and moderate-temperature extension, forming a temperature cycle. This allows the target gene to be rapidly amplified. It is characterized by strong specificity, high sensitivity, simple operation, and time-saving, and represents a major innovation in gene amplification technology. PCR technology can specifically amplify extremely small amounts of target DNA by millions of times, greatly enhancing the ability to analyze and detect DNA molecules.

Real-time quantitative PCR technology incorporates corresponding fluorescent dyes or fluorescently labeled probes based on conventional PCR. During the PCR reaction, it monitors the entire PCR process in real-time through changes in fluorescent signals. This method uses fluorescent chemicals to monitor the total amount of products after each polymerase chain reaction (PCR) cycle, and employs internal or external reference methods to quantitatively analyze specific DNA sequences in the test sample.

Basic Structure 

The real-time quantitative PCR system is an instrument for real-time detection of reactions, primarily consisting of a gene amplification thermal cycling system, a real-time fluorescence detection system, a microcircuit control system, and a computer and application software. It includes two core functional modules: the thermal cycling system and the real-time fluorescence detection system. The gene amplification thermal cycling system operates on a principle similar to that of traditional gene amplifiers, utilizing semiconductor heating and cooling to complete the thermal cycling process. The fluorescence detection system mainly consists of a fluorescence excitation component, a light signal transmission component, a fluorescence detection component, and a control system.

Feature

1. Small in size, lightweight, and easy to carry. It easily meets the needs of outdoor experiments.

2. Equipped with a 7-inch high-definition capacitive screen PDA, it allows for touchscreen operation, making it simple and fast.

3. Marlow's high-quality Peltier cooling chip, combined with Germany's high-end PT1000 temperature sensor and an electrical resistance heating compensation temperature control mode for the edges, achieves a maximum temperature rise rate of 7℃ and a maximum temperature drop rate of 5℃, significantly reducing experimental time.

4. The whole plate adopts a 3s quick lighting mode to ensure consistency of experimental results across all well positions.

5. With concise and intuitive software guidance, it is easy to start the detection experiment.

Application Field

□ Detection of waterfowl diseases

□ Poultry disease testing

□ Animal epidemic detection

□ Pathogen detection

□ Adulteration of meat products

□ Food hygiene and quarantine

□ Rational drug use and drug development

□ Gene expression

□ Water body monitoring

Technical Specifications

Sample capacity: 8x0.2ml, supports 8-tube strip

Applicable consumables: Common transparent PCR consumables, 8x0.2ml strip tubes, 0.2ml single tubes

Reaction system: 5-120ul reaction mode system

Heating/Cooling Module: Imported semiconductor thermoelectric module

Temperature control range: 4°C-99℃

The average rate of temperature rise and fall is ≥2°C/second

Temperature control accuracy: ≤±0.1°C

Temperature uniformity: ≤± 0.2°C

Number of temperature control zones: Multi-point (2 points)

Gradient count: 0

Gradient temperature range: None

Number of gradient holes: None

Excitation light source: maintenance-free LED

Excitation light wavelength range: 400-700nm

Detection component: imported photoelectric detector

Number of detection channels: Standard configuration: 1 channel (FAM); High-end configuration (optional): (FAM, VIC)

Applicable dyes and probes: FAM/SYBR Green I, VIC/HEX/CY3 (optional), ROX/Texas Red (optional), Cy5, TAMARA (optional)

Software functions: Fluorescence quantitative PCR system software; real-time amplification reaction curve function; real-time reaction curve display for specific specimens; data analysis function; automatic determination of positive and negative results; graphical display function.

Noise: <45 dB

Screen size: 7 inches (HD)

Touch screen: capacitive

External USB: Supports data import and export

Hot lid: automatic pressure adjustment

Overall dimensions: 290(W)*308(L)*130(H)

Box size: 75cm (length) * 38cm (width) * 19cm (height)

Net weight: approximately 3Kg