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Microplate washing controls liquid removal and buffer delivery between assay stages. HM-ZX01 supports programmed washing and aspiration settings. Establish the plate geometry and kit procedure before adjusting wash cycles, soak time or aspiration position.
HM-ZX01 is part of the HENG MEI Intelligent Manufacturing range supplied by Shandong Hengmei Electronic Technology Co., Ltd.
How the measurement works
HM-ZX01 removes well liquid and dispenses wash solution through an 8- or 12-needle head. Plate geometry, aspiration position, wash volume and timing determine the washing sequence required by a particular microplate assay.
Set up the analytical workflow
- Select the plate type and install the compatible washing head.
- Prime the liquid path and inspect the fluid supply and waste arrangement.
- Run the kit-specified wash program with suitable aspiration settings.
- Review controls and plate condition, then complete the prescribed rinse and shutdown sequence.
Technical configuration
| Parameter | Specification |
|---|---|
| Washing heads | 8 needles or 12 needles |
| Dispense setting | 10–3000 µL, in 10 µL increments |
| Wash count setting | 1–99 cycles |
Estimate wash-buffer demand
A 96-well plate receiving 300 µL per well uses 28.8 mL for one full dispense cycle. Five such cycles require 144 mL before priming, line filling and any additional rinse. Use the actual program volume, number of wells and cycle count to calculate the working buffer supply and waste capacity.
Use assay controls to assess the washing process
Residual unbound material can affect background, while unsuitable handling can disturb the assay material. Review controls with the configured washing sequence and plate geometry. Keep program identity and maintenance records available when investigating changes between assay batches.
Introduce a new microplate or assay
Review the well geometry and the kit washing instructions before selecting a stored program. Check head alignment and the permitted aspiration settings with the actual plate. Evaluate buffer delivery and removal together with assay control performance. Include priming and the shutdown rinse in operator training, since the liquid path must remain suitable for the next run. Save the accepted program with its plate and assay identity. When background or well-to-well behavior changes, this record helps the laboratory compare the washing conditions against those used during the original evaluation.
Select a washing program for the actual plate
Match the plate shape and assay to the washing head and program. Evaluate liquid removal, buffer delivery and residual volume with the intended consumables. Include priming, waste handling and shutdown rinsing in the operating plan. Adjust cycle count and soaking through the assay procedure and its control performance.
Investigate unexpected results
| Observed issue | Checks and follow-up |
|---|---|
| Some wells retain liquid | Inspect permitted head adjustments, needle condition and plate alignment. |
| Background increases across a plate | Review buffer supply, aspiration, washing sequence and the assay controls. |
| Flow becomes uneven | Follow the cleaning and priming procedure for the liquid path and needles. |
Keep a usable analytical record
Retain these details with the result so the preparation, measurement and subsequent review can be reconstructed.
- Plate type and assay
- Head and wash-program identity
- Buffer, cycles and soak settings
- Controls, priming and maintenance
Technical questions from operators
Should the maximum number of washes always be used?
Follow the assay procedure and evaluate performance with the actual plate and controls.
What can cause inconsistent washing?
Review head alignment, blocked needles, plate geometry, liquid supply, aspiration settings and the programmed sequence.
Use the required controls and the method settings for each sample group, and preserve the original observations alongside the accepted result and review action.
Article address:https://www.molecularbio.cn/news/microplate-washing-for-elisa-workflows.html
