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HENG MEI Intelligent Manufacturing Tray Sealing in Enzyme-Substrate Water Testing

Source:Hengmei Molecular Biology Instrument Network    Update time:2026-11-13 13:30:31

HM-FKJ Quantitative Tray Sealer on a white background

The tray sealer prepares partitioned water tests for incubation. HM-FKJ seals compatible 51-well and 97-well trays. The sealing step preserves the separate wells used for subsequent color or fluorescence observations and most-probable-number interpretation.

HM-FKJ is part of the HENG MEI Intelligent Manufacturing range supplied by Shandong Hengmei Electronic Technology Co., Ltd.

How the measurement works

HM-FKJ seals compatible 51-well and 97-well trays for enzyme-substrate water tests. After incubation, the pattern of positive wells is interpreted with the table corresponding to the tray. The complete result depends on reagent preparation, tray integrity, incubation and endpoint reading.

Set up the analytical workflow

  1. Prepare the sample with the compatible enzyme-substrate reagent.
  2. Fill and load the specified quantitative tray according to the procedure.
  3. Seal the tray and inspect its condition before incubation.
  4. Read the accepted endpoint and use the MPN table corresponding to the tray and sample dilution.

Technical configuration

ParameterSpecification
Tray formats51-well and 97-well quantitative trays
sealing time≤12 seconds per compatible tray
warm-up≤3 minutes

Include dilution in the MPN report

If the matching tray table gives 120 MPN/100 mL for a sample diluted by a factor of ten, the corresponding original-sample result is 1,200 MPN/100 mL under that reporting procedure. Keep the dilution and tray identity with the positive-well pattern. Choose additional dilution through the method when the pattern reaches the upper reporting range.

Separate sealing capacity from reporting time

HM-FKJ seals a compatible tray within 12 seconds and warms up within three minutes. Schedule incubation and endpoint reading as separate stages. After sealing, inspect the tray for the condition required by the procedure. Count positive wells consistently and use the matching table for the actual well layout and dilution.

Organize a water microbiology testing day

Set up the day around sample receipt, preparation, sealing, incubation and reading. Make sure tray labels remain readable through the full sequence and link each tray to the water sample and dilution. Arrange incubator space for the expected arrival pattern, including controls and any repeat work. Inspect sealed trays before loading and document any problem through the laboratory procedure. At the reading stage, record the well pattern before calculating the final result. Keep the matching table and dilution accessible to the reviewer. This workflow turns fast sealing into an orderly, traceable analytical process.

Purchase the complete tray-testing workflow

Specify the tray format, reagent system, incubator capacity and color or fluorescence reading arrangement together. Plan sample preparation and tray handling so loaded trays can move through sealing and incubation consistently. Include consumable availability and the reporting range needed for the expected water samples.

Investigate unexpected results

Observed issueChecks and follow-up
The sealed tray leaksReview tray compatibility, loading orientation and instrument readiness.
The positive-well pattern exceeds the table rangeApply the method procedure for dilution and repeat testing.
Endpoint reading is ambiguousReview incubation conditions, reagent controls and the specified viewing arrangement.

Keep a usable analytical record

Retain these details with the result so the preparation, measurement and subsequent review can be reconstructed.

  • Water sample and reagent lot
  • Tray format and dilution
  • Incubation start, duration and conditions
  • Positive-well pattern and MPN table

Technical questions from operators

Can 51-well and 97-well trays use the same MPN table?

Use the table specified for the actual tray format and dilution.

Does a short sealing time give an immediate result?

The assay includes incubation and endpoint reading after sealing.

Use the required controls and the method settings for each sample group, and preserve the original observations alongside the accepted result and review action.


Article address:https://www.molecularbio.cn/news/tray-sealing-in-enzyme-substrate-water-testing.html